Journal: Molecules

Article Title: MOBT Alleviates Pulmonary Fibrosis through an lncITPF–hnRNP -l- Complex-Mediated Signaling Pathway

doi: 10.3390/molecules27165336

Figure Lengend Snippet: MOBT regulated the downstream target genes of MEF2c. ( A ) A ChIP-seq experiment was performed to identify genes binding with MEF2c. ( B ) RNA-seq was performed to analyze the differentially expressed genes. ( C ) Combined analysis revealed a total of 76 target genes in the ChIP-seq and RNA-seq data, with 11 upregulated and 65 downregulated genes. ( D ) The KEGG analysis revealed that the 76 target genes regulated by MEF2c were significantly enriched in the Hippo, Wnt, and MAPK signaling pathways. ( E ) The 44 significant differentially expressed genes that were selected from 76 target genes and listed in the hierarchical clustering of RNA-seq. ( F ) The binding sites for MEF2c in the TAGLN2 and FMN1 genes were analyzed.

Article Snippet: FMN1 , 25982-1-AP , Proteintech.

Techniques: ChIP-sequencing, Binding Assay, RNA Sequencing, Protein-Protein interactions

Journal: Molecules

Article Title: MOBT Alleviates Pulmonary Fibrosis through an lncITPF–hnRNP -l- Complex-Mediated Signaling Pathway

doi: 10.3390/molecules27165336

Figure Lengend Snippet: MOBT regulated TAGLN2 and FMN1 via MEF2c. ( A ) The qRT-PCR and RNA-seq data demonstrated that TAGLN2 and FMN1 decreased under MOBT treatment compared with those in the TGF-β1 group. ( B ) The RIP experiment demonstrated that MEF2c bound to TAGLN2 and FMN1. ( C ) The Western blot result verified that MOBT inhibited TAGLN2 and FMN1. Each bar represents mean ± SD, * p < 0.05.

Article Snippet: FMN1 , 25982-1-AP , Proteintech.

Techniques: Quantitative RT-PCR, RNA Sequencing, Western Blot

Journal: Molecules

Article Title: MOBT Alleviates Pulmonary Fibrosis through an lncITPF–hnRNP -l- Complex-Mediated Signaling Pathway

doi: 10.3390/molecules27165336

Figure Lengend Snippet: Anti-pulmonary fibrotic mechanism of MOBT. MOBT inhibited lncITPF transcription by preventing p-Smad2/3 translocation from the cytoplasm to the nucleus, resulting in the reduction of the lncITPF–hnRNP L complex. The decreased lncITPF–hnRNP L complex reduced MEF2c expression by blocking its alternative splicing, which in turn inhibited the expression of MEF2c target genes, such as TAGLN2 and FMN1.

Article Snippet: FMN1 , 25982-1-AP , Proteintech.

Techniques: Translocation Assay, Expressing, Blocking Assay, Alternative Splicing

Journal: Molecules

Article Title: MOBT Alleviates Pulmonary Fibrosis through an lncITPF–hnRNP -l- Complex-Mediated Signaling Pathway

doi: 10.3390/molecules27165336

Figure Lengend Snippet: The item number and the brand of antibody.

Article Snippet: FMN1 , 25982-1-AP , Proteintech.

Techniques:

Journal: Molecules

Article Title: MOBT Alleviates Pulmonary Fibrosis through an lncITPF–hnRNP -l- Complex-Mediated Signaling Pathway

doi: 10.3390/molecules27165336

Figure Lengend Snippet: The primers used in experiments.

Article Snippet: FMN1 , 25982-1-AP , Proteintech.

Techniques: Sequencing